生物加工过程Issue(3)：52-57,6.DOI:10.3969/j.issn.1672-3678.2014.03.010
融合蛋白CBD SPA“Z”基因的构建、表达与活性鉴定
Construction,expression,and biological assay of fusion protein CBD-SPA Z
摘要
Abstract
To solve the problems of staphylococcal protein A(SPA) application,recombining the fusion protein of cellulose binding domain(CBD) with Z peptide of SPA,introducing a cysteine at the C-terminal of Z peptide, and construction and expression of immune affinity material were investigated�Prokaryotic expression vector pET35b(+)-Z-Cys was constructed by using the genetic engineering to clone the Z gene of pEZZ18 plasmid and inserted into the pET35b(+) plasmid with CBD gene,thus obtaining the fusion protein CBD-protein Z by IPTG�The plasmid pET35b(+)-Z-Cys was correctly expressed in E�coli BL21 and the fusion protein retained the bio-functional effects of Z peptide and CBD�CBD could bind to cellulose and Z peptide bind to immunoglobulin G( IgG) of many mammalians,via Fc region of IgG�关键词
纤维素结合结构域/免疫亲和层析/金黄色葡萄球菌蛋白A/Z结构域/IgG纯化Key words
cellulose binding domain/immunoaffinity chromatography/staphylococcal protein A/Z peptide/purification of IgG分类
生物科学引用本文复制引用
朱文杰,马军,张妍,金坚..融合蛋白CBD SPA“Z”基因的构建、表达与活性鉴定[J].生物加工过程,2014,(3):52-57,6.基金项目
国家大学生创新训练计划 （）
