生物加工过程Issue(4)：49-54,6.DOI:10.3969/j.issn.1672-3678.2014.04.010
共表达烟酸转磷酸核糖激酶和丙酮酸羧化酶对大肠杆菌NZN111产丁二酸的影响
Effects of co-expression of nicotinic acid phosphoribosyltransferase and pyruvate carboxylase on succinic acid production in Escherichia coli NZN111
摘要
Abstract
The recombinant plasmid pTrc99a-pncB-pyc, co-expressing nicotinic acid phosphoribosy ltransferase ( NAPRTase ) and pyruvate carboxylase ( PYC ) , was constructed and the capability of succinic acid production in E. coli NZN111/pTrc99a-pncB-pyc was investigated. The results showed that the specific enzyme activities of NAPRTase and PYC in E. coli NZN111/pTrc99a-pncB-pyc reached the highest, which were 20�75 and 1�04 U/mg, respectively. At the same time, the total amount of NADH, NAD+, and NAD ( H ) reached the highest. The fermentation results in the sealed bottles showed that 17�5 g/L glucose was consumed and 14�08 g/L was produced at 48 h and the accumulation of pyruvic acid was decreased to 0�11 g/L. The study provided a basis for the production of succinic acid in the engineered E.coli under anaerobic conditions.关键词
厌氧发酵/大肠杆菌/烟酸转磷酸核糖激酶/丙酮酸羧化酶/丁二酸Key words
anaerobic fermentation/E.coli/nicotinic acid phosphoribosyltransferase/pyruvate carboxylase/succinic acid分类
化学化工引用本文复制引用
梁丽亚,刘嵘明,苟冬梅,陈旭,马江锋,陈可泉,姜岷,韦萍..共表达烟酸转磷酸核糖激酶和丙酮酸羧化酶对大肠杆菌NZN111产丁二酸的影响[J].生物加工过程,2014,(4):49-54,6.基金项目
国家重点基础研究发展计划（973计划）（2009CB724701）；国家自然科学基金（21076105）；江苏省“青蓝工程”资助项目及江苏省优势学科；高等学校博士学科点专项基金 （）
