生物加工过程Issue(4)：87-93,7.DOI:10.3969/j.issn.1672-3678.2014.04.017
一株γ多聚谷氨酸生产菌的分离筛选与鉴定
Isolation and identification of Bacillus strain producing γ-polyglutamic acid
摘要
Abstract
Soil sample was collected from farmland. The strains were screened by the methods of gradient dilution and plate streaking on the selected medium containing glutamic acid and under the indication of the viscosity of their fermentation broth. The γ-polyglutamic acid production of the strains was obtained by analyzing the glutamic acid content of the hydrolysate of γ-polyglutamic acid using amino acid analyser. Then, the isolated strain was identified according to the morphological features, physiological and biochemical characteristics, as well as phylogenetic analysis of 16S rDNA sequences. Finally, the pgsA, pgsB, and pgsC gene, related to the γ-polyglutamic acid synthesis were amplified by PCR. A Bacillus, called the C1, was isolated with aγ-polyglutamic acid production of 18�4 g/L in flask fermentation culture. The molecular mass ofγ-polyglutamic acid produced by strain C1 was 1�8×106 detected by GPC. C1 was a gram-positive bacilli, which fermented both glucose and sucrose, hydrolysed starch, produced indole, and shared 100% sequence identity of its 16S rDNA with Bacillus amyloliquefaciens ATCC 23350, thus it was called the Bacillus amyloliquefaciens C1. It was testified to harboring pgsA, pgsB, and pgsC genes.关键词
解淀粉芽胞杆菌/鉴定/分离筛选/γ 多聚谷氨酸Key words
Bacillus amyloliquefaciens/identification/isolation/γ-polyglutamic acid(γ-PGA)分类
化学化工引用本文复制引用
何剑,雍晓雨,周俊,王舒雅,陈怡露,郑涛..一株γ多聚谷氨酸生产菌的分离筛选与鉴定[J].生物加工过程,2014,(4):87-93,7.基金项目
国家重点基础研究发展计划（973计划）（2013CB733904，2013CB7335002，2011CBA00807）；国家高技术研究发展计划（863计划）（2012AA023406）；江苏省自然科学基金（BK20130932）；江苏省高校自然科学基金 （）
