生物加工过程Issue(5)：23-28,6.DOI:10.3969/j.issn.1672-3678.2014.05.004
产朊假丝酵母全细胞转化合成谷胱甘肽的营养条件
Media optimization for glutathione production by bioconversion using Candida utilis as whole-cell catalyst
摘要
Abstract
Candida utilis SZU 07-01 was used as a whole-cell catalyst for the production of glutathione ( GSH) by bioconversion. In order to improve the capability of the yeast cell on GSH biotransformation,the response surface methodology was applied for the media optimization. Based on the results of the single-factor experiments together with the Plackett-Burman design, glucose and KH2 PO4 were selected as the primary factors with significant influences on GSH biotransformation rate. The optimal concentrations of 58�5 and 17�2 g/L for glucose and KH2 PO4 were predicted by using the steepest ascent test and the response surface methodology,respectively�According to the verification experiments in flasks,the average GSH biotransformation rate of 1�54 mg/( g·h ) was achieved under the optimized medium, which was increased by 100%compared with that without media optimization. The results provided a feasible approach on the efficient production of analogical useful chemicals synthesized by the whole-cell bioconversion.关键词
谷胱甘肽/全细胞转化/产朊假丝酵母/响应面分析Key words
glutathione/whole-cell conversion/Candida utilis/response surface methodology分类
生物科学引用本文复制引用
朱健,胥京京,汪成富,卫功元..产朊假丝酵母全细胞转化合成谷胱甘肽的营养条件[J].生物加工过程,2014,(5):23-28,6.基金项目
国家自然科学基金 （）
