生物加工过程Issue(5)：39-45,7.DOI:10.3969/j.issn.1672-3678.2014.05.007
Cre／LoxP重组系统的改造及在树干毕赤酵母中的应用
Modification of Cre/LoxP recombination system and its application in Pichia stipitis
摘要
Abstract
Heterologous genes rich in CTG could not express in P�stipitis. A modified Cre/LoxP recombination system was developed to knock out the genes in P�stipitis with no trace. Firstly,ura3 was deleted to obtain a uracil auxotroph; Secondly, CTG codons in hpt and cre of pSH47-Hpt were mutagenized to TTG;Finally,the modified Cre/LoxP recombination system was applied to knock out the genes without trace in P�stipitis using acetaldehyde dehydrogenase gene as a target gene. In this study, Hygromycin B resistant marker was referred to,and the modified Cre/LoxP recombination system could knock out the target gene with no trace in P�tsipitis, which provided a method and a new resistance marker for the further study of functional genes and metabolic pathway in P�stipitis.关键词
树干毕赤酵母/基因敲除/Cre/LoxP重组/潮霉素BKey words
Pichia stipitis/gene knockout/Cre/LoxP recombination/hygromycin B分类
生物科学引用本文复制引用
傅静,张梁,薛卫,石贵阳..Cre／LoxP重组系统的改造及在树干毕赤酵母中的应用[J].生物加工过程,2014,(5):39-45,7.基金项目
江苏省自然科学基金（ BK2011153，BK2012363）；教育部“新世纪优秀人才支持计划” （）
