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1株产细菌纤维素芽胞杆菌的分离及鉴定

朱宏阳 姚俊 冯珊 李泳宁 林伟铃

生物加工过程Issue(5):80-84,5.
生物加工过程Issue(5):80-84,5.DOI:10.3969/j.issn.1672-3678.2014.05.014

1株产细菌纤维素芽胞杆菌的分离及鉴定

Isolation and identification of bacterial cellulose producing strain

朱宏阳 1姚俊 2冯珊 1李泳宁 1林伟铃1

作者信息

  • 1. 福建卫生职业技术学院 药学系 福州,350101
  • 2. 南京医科大学 基础医学院 南京 210029
  • 折叠

摘要

Abstract

Strain ZF-7 was isolated from bad fruits. Its product was determined as bacterial cellulose ( BC) in view of the results of cellulose special reaction, FT-IR spectroscopy analysis and cellulose hydrolysis experiments. Based on morphology, physiological and biochemical characteristics, ZF-7 was identified by 16S rDNA sequences and systematic analysis. The results indicated that 16S rDNA sequences of ZF-7 had 99�5% similarity to the partial sequence of Bacillus amyloliquefaciens,suggesting that ZF-7 was categorized as a subspecies of B�amyloliquefaciens, and denominated as B�amyloliquefaciens ZF-7�The fermentation performance of ZF-7 under shaking and static flasks was inspected preliminarily,and the BC production was 6�6 and 6�2 g/L,respectively�

关键词

细菌纤维素/解淀粉芽胞杆菌/筛选/鉴定

Key words

bacterial cellulose/Bacillus amyloliquefaciens/isolation/identification

分类

生物科学

引用本文复制引用

朱宏阳,姚俊,冯珊,李泳宁,林伟铃..1株产细菌纤维素芽胞杆菌的分离及鉴定[J].生物加工过程,2014,(5):80-84,5.

基金项目

福建省自然科学青年基金(2012J05015);福建省教育厅A类科技项目 ()

生物加工过程

OACSTPCD

1672-3678

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