生物加工过程Issue(6)：6-12,7.DOI:10.3969/j.issn.1672-3678.2014.06.002
响应面法优化α蒎烯发酵培养基
Medium optimization for α-pinene production with response surface analysis
摘要
Abstract
Response surface analysis was used to optimize for α⁃pinene production. Initially, MD beef extract powder was determined to be the best nitrogen source for α⁃pinene production, then Plackett⁃Burman ( P⁃B ) experiment was used to evaluate the eight nutritional variables to screen medium component that significantly influence the α⁃pinene production and their optimal levels. Results showed that beef extract powder,pH and citric acid were significant factors. Then the path of steepest ascent was used to approach the optimal region of the above three factors. In the third step,the concentration of beef extract powder,pH and citric acid was further optimized using central composite designs and response surface analysis. As the result,the concentration ofα⁃pinene increased from 1�710 to 7�053 mg/L. With the optimized medium,the yield of α⁃pinene increased about 3⁃fold.关键词
大肠杆菌/α 蒎烯/优化/响应面分析Key words
E�coli/α-pinene/optimization/response surface analysis分类
生物科学引用本文复制引用
任萌,杨建明,咸漠..响应面法优化α蒎烯发酵培养基[J].生物加工过程,2014,(6):6-12,7.基金项目
国家自然科学基金（21376255）；国家高技术研究发展计划（863计划）（SS2013AA050703-2）；青岛科技发展计划（12-1-4-9-（3）-jch） （）
