生物加工过程Issue(6)：57-61,5.DOI:10.3969/j.issn.1672-3678.2014.06.011
离子交换层析结合反向色谱纯化聚二氨基丙酸
Purification of poly (L-diaminopropionic acid) by ion-exchange and reversed-phase chromatography
摘要
Abstract
The ε⁃polylysine producing strain Streptomyces albulus PD⁃1 could synthesize a novel non⁃proteinic amino acid oligomer:poly ( L⁃diaminopropionic acid ) ( PDAP )�Ion⁃exchange and reversed⁃phase chromatography were used to study the purification conditions of PDAP�In the ion⁃exchange chromatography purification,DEAE⁃Sepharose Fast Flow was selected,50 mmol/L phosphate buffer was used as mobile phase and 0�5 mol/L NaCl in the phosphate buffer acted as elution�In the reversed⁃phase chromatography purification,ratio of methanol to 0�1% phosphoric acid was 5/95 ( V/V) and was used as the mobile phase�Pure PDAP sample was obtained by ion⁃exchange and reversed⁃phase chromatography, the recovery rate of the purification was 39�8%,and the purity of the sample was 98.4%.关键词
聚二氨基丙酸/纯化/离子交换层析/反向色谱Key words
poly( L-diaminopropionic acid)/purification/ion-exchange chromatography/reversed-phase chromatography分类
化学化工引用本文复制引用
夏军,许召贤,薄芳芳,冯小海,迟波,徐虹..离子交换层析结合反向色谱纯化聚二氨基丙酸[J].生物加工过程,2014,(6):57-61,5.基金项目
国家自然科学基金（21176123、21006050）；国家重点基础研究发展计划（973计划）（2013CB733600）；高等学校博士学科点专项科研基金（20113221130001）；长江学者和创新团队发展计划（IRT1066）；江苏省普通高校研究生科研创新计划 （）
