生物加工过程Issue(6)：62-66,95,6.DOI:10.3969/j.issn.1672-3678.2014.06.012
化学抑制剂Woodward′s Reagent K对来源于Erwinia rhapontici NX 5蔗糖异构酶的抑制动力学
Inhibition kinetics of sucrose isomerase from Erwinia rhapontici NX-5 by Woodward′s Reagent K
摘要
Abstract
Sucrose isomerase ( SIase,EC5�4�99�11) from Erwinia rhapontici NX⁃5 was purified from the extract of recombinant E�coli BL21 ( pET22b⁃palⅠ) culture,and the inhibition kinetics of the pure SIase was studied with chemical inhibitor Woodward′s Reagent K( WRK)�Results show that SIase had the high specific activity of 1 512�77 U/mg,as well as the Michaelis⁃Menten constants of Km=260 mmol/L and Vmax=39�41 μmol/( L·s)�Km increased as the concentration of inhibitor increased,but Vmax kept stable within limits�The inhibition of SIase by WRK was reversible and competitive, probably caused by the similar structure of WRK and sucrose.关键词
蔗糖异构酶/抑制动力学/化学抑制剂Woodward′s Reagent KKey words
sucrose isomerase/inhibition kinetics/Woodward′s Reagent K分类
生物科学引用本文复制引用
王彦媛,李莎,姚忠,徐虹..化学抑制剂Woodward′s Reagent K对来源于Erwinia rhapontici NX 5蔗糖异构酶的抑制动力学[J].生物加工过程,2014,(6):62-66,95,6.基金项目
国家高技术研究发展计划（863计划）（2012AA021503）；国家自然科学基金（31371732）；教育部博士点基金（20103221110006）；江苏省普通高校研究生科研创新计划 （）
