生物加工过程Issue(6)：78-83,6.DOI:10.3969/j.issn.1672-3678.2014.06.015
尼罗红荧光法快速检测培养过程中湛江等鞭金藻的中性脂
Nile red fluorescence method for rapid measurement of neutral lipids during the cultivation of the marine microalgae Isochrysis zhangjiangensis
摘要
Abstract
A rapid method for quantifying neutral lipids in microalgae is impending�The marine microalga Isochrysis zhangjiangensis, an ideal candidate for producing biodiesel, was taken as a model system to establish the nile red florescence method�The optimal staining condition was 2�0% dimethyl sulfoxide (V/V),1�00 μg/mL nile red,1�0×106 cell/mL cell density and staining for 10 min�The excitation and emission wavelength was 480 nm and 580 nm,respectively�Background fluorescence at different growth phase was measured and its intensity was only about 20% of the florescence intensity in cell�Compared with the fluorescence intensity in cell,the intensity of background florescence was weak and negligible�A correlation coefficient of R2=0�946 8 was obtained between the fluorescence intensity of nile red and the neutral lipid content measured by conventional gravimetric method�Although the correlation coefficient was not perfect, nile red method is useful in determining neutral lipid content during microalga cultivation.关键词
尼罗红/中性脂/湛江等鞭金藻/生物柴油Key words
nile red/neutral lipid/Isochrysis zhangjiangensis/biodiesel分类
生物科学引用本文复制引用
王海涛,刘亚男,曹旭鹏,薛松,王伟良..尼罗红荧光法快速检测培养过程中湛江等鞭金藻的中性脂[J].生物加工过程,2014,(6):78-83,6.基金项目
国家重点基础研究发展计划（973计划）（2011CB200903）；国家高技术研究发展计划（863计划）（2012AA052101）；辽宁省自然科学基金（2012010263）；中国科学院“百人计划” （）
