生物加工过程Issue(1)：1-5,5.DOI:10�3969/j�issn�1672-3678�2015�01�001
Gibberella intermedia CA31羟基化去氢表雄酮的工艺
C7α-hydroxylation of dehydroepiandrosterone by Gibberella intermedia CA3-1
摘要
Abstract
We studied enzymatic C7⁃hydroxylation of dehydroepiandrosterone ( DHEA ) by Gibberella intermedia CA3⁃1. The single factor experiment was applied to determine the optimal fermentation conditions with the conversion rate of C7⁃hydroxylation as the criterion. The optimal biotransformation conditions were as follows:inoculation amount 6% ( V/V) , medium volume 30 mL ( per 250 mL flask) , rotation speed 220 r/min, DHEA concentration 1 g/L, co⁃solvent propylene glycol (6%, V/V), adding substrate at the same time with inoculation, and biotransformation time 36 h. The molar yield of 7α⁃OH⁃DHEA from substrate DHEA was 72�34%.关键词
生物转化/7α-羟基化/去氢表雄酮/甾体/Gibberella intermediaKey words
biotransformation/7α-hydroxylation/dehydroepiandrosterone/steroid/Gibberella intermedia分类
化学化工引用本文复制引用
付珍珍,李恒,李会,许正宏..Gibberella intermedia CA31羟基化去氢表雄酮的工艺[J].生物加工过程,2015,(1):1-5,5.基金项目
国家高技术研究发展计划（863计划）（2011AA02A211）；国家自然科学基金（21206055）；江苏省自然科学基金 （）
