生物加工过程Issue(1)：261-264,4.DOI:10.3969/j.issn.1673-7202.2015.02.028
高山大黄的显微及分子鉴定研究
Microscopic and Molecular Identification of Rheum Nobile
摘要
Abstract
Objective:To perform a study of the medicinal herbal microscopic and molecular identification of Rheum nobile radix and rhizome.Methods:The cross sections of Rheum nobile radix and rhizome were obtained by using paraffin sectioning,and tem-porary glycerol tablets with chloral hydrate were used to identify the microscopic structure of powder.The matK gene sequence anal-ysis technique was used for molecular identification of Rheum nobile.Results:In the cross-section of radix,both the cortex and xy-lem were wide with vessels arranging radially and massive calcium oxalate crystal visible in parenchyma cells.Pith part was broad without any abnormal vascular bundle.Powder contained a mass of calcium oxalate crystals whose edges were mostly short blunt. There were lignified vessels and small starch grain as well.The length of matK gene sequence of Rheum nobile was 1 524 bp.Up-stream of matK sequence,there were ten base-pair substitutions in 1 06,21 8,222,365,522,61 5,71 1 ,759,801 ,852,and 6 bases CAAGAA inserted in 950-955.Conclusion:The research tends to contain relatively comprehensive identification information of Rheum nobile.These microscopic features can be used as evidence for identification of Rheum nobile radix and rhizome,and the matK gene sequence analysis helps lay a foundation for Rheum nobile DNA barcoding research.关键词
高山大黄/显微鉴定/分子鉴定Key words
Rheum nobile/Microscopic identification/Molecular identification分类
医药卫生引用本文复制引用
费烨,付深振,吴浩忠,李启恩,多吉任青,陈静,闫兴丽,魏胜利,高增平..高山大黄的显微及分子鉴定研究[J].生物加工过程,2015,(1):261-264,4.基金项目
北京中医药大学自主选题（藏医药类）课题资助项目 （）
