生物加工过程Issue(1)：54-59,6.DOI:10�3969/j�issn�1672-3678�2015�01�010
以甘油为底物鼠李糖脂高产菌株的诱变选育
Mutating rhamnolipids high-yielding strain with glycerol as substrate
摘要
Abstract
Through mutation, rhamnolipids yield of Pseudomonas aeruginosa RG⁃14 increased from 13�6 g/L to 16�5 g/L. After 5 rounds of subculturing, rhamnolipids yield of the mutant stayed stable. Subsequently, MALDI⁃TOF⁃MS was applied to analyze the composition of rhamnolipids produced by the mutant. The results showed that the rhamnolipids congener was comprised of Rha⁃C8⁃C8,Rha⁃C8⁃C10, Rha⁃C10⁃C10, Rha⁃C10⁃C12 ∶ 1, Rha⁃C10⁃C12, Rha2⁃C8⁃C10, Rha2⁃C10⁃C10, Rha2⁃C10⁃C12 ∶ 1 and Rha2⁃C10⁃C12. The relative abundance of mono⁃rhamnolipids and di⁃rhamnolipids were 54�8% and 45�2%, respectively. The mutant strain also could use crude glycerol besides refined glycerol, and the rhamnolipids yield reached 14�2 g/L, showing application potential of this strain.关键词
铜绿假单胞菌/鼠李糖脂/亚硝基胍诱变/粗甘油Key words
Pseudomonas aeruginosa/rhamnolipid/NTG-induced mutation/crude glycerol分类
生物科学引用本文复制引用
顾生辉,朱莉,詹晓北,吴剑荣..以甘油为底物鼠李糖脂高产菌株的诱变选育[J].生物加工过程,2015,(1):54-59,6.基金项目
国家科技支撑计划 （）
