生物加工过程Issue(2)：24-29,6.DOI:10.3969/j.issn.1672-3678.2015.02.005
产花生四烯酸油脂高山被孢霉干法造粒提油及菌粕的重复利用
Extraction of lipids from arachidonic acid-rich oil producing Mortierella alpina with dry granulation and reusing fungal residues
摘要
Abstract
Extracting lipids from arachidonic acid⁃rich oil producing Mortierella alpina with dry granulation and fungal residues reuse methods were developed. The effects of three parameters, including fungal particle diameter, proportion of n⁃hexane⁃to⁃fungal residues and extraction time, on the lipid extraction efficiency and n⁃hexane recovery rate were investigated through L16(43) orthogonal experiment. When the moisture content of fungal particles was between 20% and 25%,with the aforementimed three parameters being 1�2 mm,20∶1 L/g and 5 h,respectively,the lipid and n⁃hexane recovery yield reached 0�559 4 g/g dry biomass and 90%,increased by 17�35% and 83�67%,comparing with the traditionally used homogeneous wet extraction method. Pretreating with alcalase and complex enzyme including cellulose,pectinase and alcalase,fungal residues could be resued to replace the nitrogen source partly for the growth of M. alpina. When 50% proportion of fungal residues were added into the medium, the fermentation time was 10 d with alcalase pretreatment and 7 d with complex enzyme pretreatment. At the same time, biomass, lipid and ARA content reached 30�33 g/L, 16�25 g/L, 8�59 g/L ( alcalase pretreatment) ,and 29�77 g/L,16�89 g/L and 7�12 g/L ( complex enzymatic pretreatment) ,respectively. The productivity of fungal residues pretreated with compound enzyme could reach 4�253, 2�413 and 1�017 g/( L·d ) , respectively. Comparing with pretreating by alcalase, the productivity increased by 40�22%,48�49% and 18�39%,respectively. Extraction with dry grandation is effective and pretreatment of fungal residue with enzyme is also promising.关键词
花生四烯酸/高山被孢霉/干法造粒/菌粕/生产强度Key words
arachidonic acid/Mortierella alpina/dry granulation/fungal residues/productivity分类
轻工纺织引用本文复制引用
商静生,纪晓俊,聂志奎,张瑷珲,颜佳铖,张鑫,黄和..产花生四烯酸油脂高山被孢霉干法造粒提油及菌粕的重复利用[J].生物加工过程,2015,(2):24-29,6.基金项目
国家高技术研究发展计划（863计划）（2014AA021703）；国家自然科学基金（21376002、21476111）；江苏省自然科学基金（ BK20131405）；江苏省高校优势学科建设工程 （）
