生物加工过程Issue(2)：30-34,5.DOI:10.3969/j.issn.1672-3678.2015.02.006
β半乳糖苷酶酶学性质及其在低聚半乳糖合成中的应用
Application of β-galactosidase for the synthesis of galactooligosaccharides
摘要
Abstract
The properties of β⁃galactosidase from Kluyveromyces lactis and the enzymatic synthesis of galactooligosaccharides ( GOS) were investigated. GOS ( degree of polymerization n>3) concentration was analyzed by high performance liquid chromatography ( HPLC ) and used as experimental index. The influences of temperature, pH, and the type and concentration of metal ions on β⁃galactosidase activity were evaluated. In addition,the effects of lactose concentration,reaction time and enzyme loading on the formed GOS concentration were optimized in the presence of K+. K+ and NH+4 could activate transglycosidase and β⁃galactosidase. However,Na+ could activate transglycosidase and inhibit hydrolase activity. Furthermore,the optimal conditions for enzymatic synthesis of GOS were determined as 37℃,pH 8,0�08 mol/L of K+,intial lactose concentration of 500 g/L,and the enzyme concentration of 10 μL/g lactose. Under these conditions,the maximum GOS concentration reached 94�74 g/L after 5 h.关键词
β 半乳糖苷酶/乳酸克鲁维酵母/低聚半乳糖Key words
β-galactosidase/Kluyveromyces lactis/galactooligosaccharides分类
轻工纺织引用本文复制引用
邢肖肖,齐崴,王梦凡,尤生萍,苏荣欣,何志敏..β半乳糖苷酶酶学性质及其在低聚半乳糖合成中的应用[J].生物加工过程,2015,(2):30-34,5.基金项目
国家自然科学基金 （）
