生物加工过程Issue(2)：81-85,5.DOI:10.3969/j.issn.1672-3678.2015.02.016
分析紫杉醇与三尖杉宁碱的简易方法
Simplified analysis and purification of paclitaxel and cephalomannine
摘要
Abstract
A simple analytical and purification method for paclitaxel and cephalomannine was developed using the MKF⁃DK⁃RP analytical column ( 300 mm × 7�8 mm, 8 μm particle size ) with monodisperse polystyrene reverse⁃phase chromatography. The mobile phase was acetonitrile⁃water at a flow rate of 1�0 mL/min. The detection wavelength was 229 nm�Paclitaxel and cephalomannine could be effectively purified. It could quantitatively analyze and purify paclitaxel in the range between 0�062 5 and 2�0 mg/mL( r=0�999 9) . The optimized process is easy to operate,and reproducible.关键词
聚苯乙烯反相色谱填料/紫杉醇/三尖杉宁碱Key words
polystyrene reverse-phase chromatography packing/Paclitaxel/Cephalomannine分类
化学化工引用本文复制引用
黄艳,刘晓宁,方芳,徐艳,魏荣卿,郑涛..分析紫杉醇与三尖杉宁碱的简易方法[J].生物加工过程,2015,(2):81-85,5.基金项目
国家重点基础研究发展计划（973计划）（2013CB733504）；国家高技术研究发展计划（863计划） （）
