生物加工过程Issue(3)：26-30,5.DOI:10.3969/j.issn.1672-3678.2015.03.005
米根霉交替呼吸途径高活性突变株的选育及其对富马酸合成的影响
Screening of Rhizopus oryzae mutants with higher activity of alternative respiration for fumaric acid production
摘要
Abstract
During fumaric acid fermentation, alternative respiration is associated with fumaric acid productivity in Rhizopus oryzae�S⁃1 mutant with higher activity of alternative respiration was screened by atmospheric and room temperature plasma ( ARTP ) ion mutagenesis using Rhizopus oryzae F⁃14 as the original strain. The activity of alternative respiration in S⁃1mutant is 3�5⁃fold than that of F⁃14. However, fumaric acid production ( 28 g/L ) was much lower than that of the original strain ( 42 g/L ) . The relationship between alternative respiration ratio and fumaric acid productivity, the change of total respiration activity and NADH/NAD+of S⁃1 and F⁃14 in the fermentation process were further studied. At the initial fermentation stage higher activity of alternative respiration in S⁃1 mutant reduced the fumaric acid productivity,and ultimately led to the premature aging phenomenon in the fermentation. It means that the activity of alternative respiration should adapt the fumaric acid productivity.关键词
米根霉/富马酸/交替呼吸/生产强度Key words
Rhizopus oryzae/fumaric acid/alternative respiration/productivity分类
能源科技引用本文复制引用
孙小龙,付永前,徐晴,李霜,黄和..米根霉交替呼吸途径高活性突变株的选育及其对富马酸合成的影响[J].生物加工过程,2015,(3):26-30,5.基金项目
国家重点基础研究发展计划（973计划）（2013CB733605）；国家高技术研究发展计划（863计划）重点项目（2011AA02A206）；国家自然科学基金 （）
