生物加工过程Issue(4)：1-6,22,7.DOI:10�3969/j�issn�1672-3678�2015�04�001
高山被孢霉发酵产花生四烯酸油脂的补料工艺
Arachidonic acid-rich oil production by Mortierella alpina using different substrate feeding strategies
摘要
Abstract
To shorten the production cycle of arachidonic acid ( ARA )⁃rich oil and increase the productivity,we studied the effects of different substrate feeding strategies on the production of ( ARA)⁃rich oil by Mortierella alpina. Batch fermentation and residual glucose feedback fed⁃batch fermentation were studied, as well as a repeated fed⁃batch fermentation that was developed on the basis of residual glucose feedback fed⁃batch. Compared with batch fermentation, ARA productivity in residual glucose feedback fed⁃batch fermentation increased from 0�93 to 1�33 g/( L·d) . Four batches were carried out for repeated fed⁃batch culture, in which the biomass remained stable around 30 g/L and lipid contents remained stable around 50%. ARA contents in total fatty acid were 42�81%, 43�17%, 42�30%, and 39�71%,respectively.关键词
高山被孢霉/花生四烯酸油脂/补料分批发酵/反复补料分批发酵Key words
Mortierella alpina/arachidonic acid-rich oil/fed-batch/repeated fed-batch分类
生物科学引用本文复制引用
聂志奎,纪晓俊,商静生,颜佳铖,张瑷珲,黄和..高山被孢霉发酵产花生四烯酸油脂的补料工艺[J].生物加工过程,2015,(4):1-6,22,7.基金项目
国家自然科学基金（21376002，21476111）；江苏省自然科学基金（BK20131405）；国家科技支撑计划（2011BAD23B03）；江苏高校优势学科建设工程 （）
