生物加工过程Issue(4)：17-22,6.DOI:10�3969/j�issn�1672-3678�2015�04�004
树脂固定化β半乳糖苷酶制备低聚半乳糖
Galactooligosaccharides production by β-galactosidase immobilized onto resins
摘要
Abstract
We used resin as a carrier to immobilize β⁃galactosidase for the production of galactooligosaccharides. Cation exchange resin with the highest absorption rate and recover rate was selected as the carrier. The immobilization condition was optimized through cross⁃linking after immobilization of β⁃galactosidase.The best condition was enzyme load 51�8 U/g resin,pH 6�5,25 ℃, time 12 h;and the concentration of glutaraldehyde of 4%( v/v); the temperature in cross⁃linking action was 40 ℃,the time was 6 h. Under the conditions, the activity of immobilized enzyme was 16�2 U/g resin, the recovery of enzyme fixed was 39�1%, the galactooligosaccharides ( GOS ) production was 24�2%. The method provides technical basis for continuous production of GOS.关键词
β 半乳糖苷酶/固定化/低聚半乳糖/树脂Key words
β-galactosidase/immobilized/galactooligosaccharides/resin分类
生物科学引用本文复制引用
费俊杰,李冰冰,陈勇,应汉杰..树脂固定化β半乳糖苷酶制备低聚半乳糖[J].生物加工过程,2015,(4):17-22,6.基金项目
国家杰出青年基金（21025625）；国家高技术研究发展计划（863计划）（2012AA021203）；国家支撑计划（2012BAIG4001）；国家自然科学基金青年基金（21106070）；长江学者和创新发展计划（PCSIRT）；江苏省高校优势学科建设工程；江苏自然科学基金 （）
