生物加工过程Issue(4)：23-28,6.DOI:10�3969/j�issn�1672-3678�2015�04�005
重组大肠杆菌共表达亮氨酸脱氢酶和甲酸脱氢酶发酵条件优化
Optimization of fermentation conditions of recombinant E coli for coexpression of leucine dehydrogenase and formate dehydrogenase
摘要
Abstract
The fermentation conditions of recombinant E�coli for coexpression of leucine dehydrogenase and formate dehydrogenase were optimized in shake flasks. The effects of carbon and nitrogen source and their concentrations, as well as initial pH of medium on the cell growth and enzyme production were studied. The optimal carbon source was glycerol ( 10 g/L ) and nitrogen source was yeast extract ( 10 g/L) . The optimal initial pH of fermentation medium was 8�0. The induction conditions were further optimized as follows:induction occasion OD600 0�6,induction temperature 25 ℃,IPTG concentration 0�2 mmol/L and induction time 20 h. Under the optimized conditions,a high cell density( OD600 ) of 8�6 was obtained. The enzyme activity of LeuDH reached 1 543�3 U/L and that of FDH reached 2 572�4 U/L, which were 2�0⁃fold and 3�1⁃fold as that before optimization.关键词
重组大肠杆菌/亮氨酸脱氢酶/甲酸脱氢酶/诱导Key words
recombinant E�coli/leucine dehydrogenase/formate dehydrogenase/induction分类
生物科学引用本文复制引用
刘维明,杨震炯,罗积杏,壮晓健,沈文和,胡燚,黄和..重组大肠杆菌共表达亮氨酸脱氢酶和甲酸脱氢酶发酵条件优化[J].生物加工过程,2015,(4):23-28,6.基金项目
国家自然科学基金杰出青年基金（21225626）；国家高技术研究发展计划（863计划）（2011AA02A209）；江苏高校优势学科建设工程 （）
