生物加工过程Issue(4)：47-51,5.DOI:10�3969/j�issn�1672-3678�2015�04�009
脂肪酶YCJ01拆分对位取代α苯乙醇
Asymmetric transesterification resolution of para-(R,S)-α-phenylethanol by lipase YCJ01
摘要
Abstract
Asymmetric transesterification resolution of para⁃( R, S )⁃α⁃phenylethanol was conducted by lipase YCJ01. The resolution conditions of para⁃(R,S)⁃α⁃phenylethanol were optimized. Reaction was carried out in isopropyl ether using vinyl acetate as acyl donors with 5 g/L lipase YCJ01 and 180 mmol/L 1⁃(4⁃methylphenyl)ethanol. The mixture was incubated at 50℃ for 21 h. After the reaction,the substrate conversion could reach 49�96% with e�e.s=97�1%,e�e.p=97�2%,E>200�Similarly,high enantiomeric purity(e�e.s=97�7%,e�e.p=98�4%,E>200) and substrate conversion(49�82%) were achieved at 30℃ after 12 h in isopropyl ether with 2�5 g/L lipase YCJ01 and 150 mmol/L 1⁃( 4⁃methoxyphenyl ) ethanol, vinyl butyrate as acyl donors. All results illustrated that lipase YCJ01 was an exploitable biocatalyst for chiral resolution of pharamaceutical intermediate.关键词
耐有机溶剂脂肪酶/手性拆分/Burholderia ambifaria/α 苯乙醇Key words
organic solvent tolerant lipase/chiral resolution/Burholderia ambifaria/α-phenylethanol分类
生物科学引用本文复制引用
秦燕,李文豪,汪斌,何冰芳..脂肪酶YCJ01拆分对位取代α苯乙醇[J].生物加工过程,2015,(4):47-51,5.基金项目
国家高技术研究发展计划（863计划）重大项目（2012AA022205）；国家重点基础研究发展计划（973计划） （）
