生物技术通报Issue(6)：67-74,8.
未培养技术在瘤胃产甲烷菌群研究中的应用
The Application of Uncultured Methods in the Study of Ruminal Methanogen Population
摘要
Abstract
The ruminal methanogen are strictly anaerobic, which are hard to culture.The ruminal methanogen which have been cultured successfully are only 4 species.while hundreds of species of methanogen could not be cultured.The application and development of uncultured technology promotes the research of ruminal methanogen, which overcome the limitation of the tranditional technology of isolation and culturing. Several dominant culture-independent technologies used to study ruminal methanogens were introduced in this paper to provide references on methods for future ruminal methanogen study.关键词
瘤胃/产甲烷菌/未培养技术/全基因组测序/高通量测序Key words
Rumen/Methanogen/Uncultured methods/Whole genome sequencing/High throughput DNA sequencing引用本文复制引用
王兴文,王加启,赵圣国,李发弟,卜登攀..未培养技术在瘤胃产甲烷菌群研究中的应用[J].生物技术通报,2014,(6):67-74,8.基金项目
国家自然科学基金项目（31261140365），科技支撑计划项目（2012BAD12B02），中国科学院知识创新工程重要方向项目 （）
