生物技术通报Issue(6)：139-144,6.
三角帆蚌四核苷酸重复微卫星的开发及特征分析
Development and Characteristics of Tetranucleotide Repeat Microsatellite Loci in Hyriopsis cumingii
摘要
Abstract
A microsatellite-enriched library of the Hyriopsis cumingii was constructed using repeat-enrichment method with biotin-labeled oligo(ATAC)5 and streptavidin coated magnetic beads. Forty-three pairs of primers designed by software PrimerSelect according to the sequences were synthesized for the pre-screening. Twenty pairs of primers which could amplify constant and legible expected DNA products were labeled by fluorescence. Seventeen microsatellite loci showed high levels in genetic polymorphism testing on 36 individuals sampled from Dongting Lake. The number of alleles at each locus ranged from 6 to 28. The expected and observed heterozygosities varied from 0.677 6 to 0.946 4 and from 0.638 9 to 1.000, respectively. The PIC value ranged from 0.630 to 0.929. Twelve microsatellite loci fitted to Hardy-Weinberg equilibrium. Compared with the previous dinucleotide repeat microsatellite developed in our laboratory, these 17 tetranucleotide repeat microsatellite loci showed higher polymorphism. Also, these 17 microsatellite loci can make genotyping easier by conventional methods such as polyacrylamide gel electrophoresis and save cost. Therefore, it offered accurate and inexpensive microsatellite markers for population genetic analysis and paternity test in Hyriopsis cumingii.关键词
三角帆蚌/微卫星/四核苷酸重复/多态性Key words
Hyriopsis cumingii/Microsatellite/Tetranucleotide repeat/Polymophism引用本文复制引用
韩学凯,李家乐,王照旗,白志毅..三角帆蚌四核苷酸重复微卫星的开发及特征分析[J].生物技术通报,2014,(6):139-144,6.基金项目
上海市高校知识服务平台项目（ZF1206），国家科技支撑计划项目（2012BAD26B04），国家自然科学基金项目（31272657），上海市青年科技启明星（A类）（12QA1401400），上海工程技术中心能力提升项目 （）
