生物技术通报Issue(6)：162-167,6.
油茶内生拮抗细菌Y13的定殖能力及其对土著细菌的影响
Colonization of Bacillus subtilis Y13 in Camellia oleifera Leaves and Its Effect on Native Bacteria
摘要
Abstract
The colonization capacity of strain Y13 in the Camellia oleifera, was investigated by marking with rifampicin, periodical sampling, and recovering bacteria through diluting-plate method, as well as the effects of Y13 on native bacterial population were also studied by diluting-plate and 16S rDNA methods, which would provide a basis for ecological control of camellia anthracnose. The results showed strain Y13R displayed good colonization in camellia oleifera, 30 days after inoculation, 6.60×103CFU/g and 3.56×103CFU/g strains Y13R could be detected in healthy and diseased leaves, respectively. Endophytic bacteria isolated from Camellia oleifera belong to 5 genera of 7 species, Bacillus, Lysinibacillus, Klebsiella, Sphingomonas and Pseudomonas, Bacillus and Lysinibacillus was the most prevalent genera. The group and amount of predominant species had evident distinction in healthy and diseased leaves. The diversities of bacteria in the Camellia oleifera leveas were improved by Y13 treatment and suppressed the pathogen propagation.关键词
油茶炭疽病/内生拮抗细菌/定殖/土著细菌Key words
Camellia anthracnose/Endophytic antagonistic bacteria/Colonization Native bacteria引用本文复制引用
王瑞芹,周国英,刘君昂,李冬琴,孟庆敏..油茶内生拮抗细菌Y13的定殖能力及其对土著细菌的影响[J].生物技术通报,2014,(6):162-167,6.基金项目
国家自然科学基金项目 （）
