生物技术通报Issue(6)：168-172,5.
红树林土壤微生物宏基因组文库的构建及两种新颖的β-葡萄糖苷酶基因的鉴定
Construction of a Mangrove Soil Metagenome Library and Identification of Two Novelβ-Glucosidase Genes
摘要
Abstract
Metagenomics provides a means of exploring new enzymes in microorganisms. A mangrove soil metagenome library with 100 000 clones was constructed. The average length of inserted DNA fragment is about 30 kb, and the total capacity of inserted DNA reached about 3 Gb. Seventeen clones withβ-glucosidase activity were detected by screening 10 000 fosmid clones. The subcloning and sequencing revealed two novelβ-glucosidase genes(MhGH3 and bgl66). Bioinformatics analysis indicated that MhGH3 and bgl66 composed of 1 992 bp and 2 025 bp in length, respectively. The deduced amino acid sequence of MhGH3 and bgl66 showed the highest sequence identity of 64%and 55%with the knownβ-glucosidase in GenBank datebase, respectively.关键词
β-葡萄糖苷酶/纤维素酶/红树林/宏基因组文库Key words
β-Glucosidase/Cellulase/Mangrove/Metagenomic library引用本文复制引用
麦志茂,苏宏飞,李丽珍,张偲..红树林土壤微生物宏基因组文库的构建及两种新颖的β-葡萄糖苷酶基因的鉴定[J].生物技术通报,2014,(6):168-172,5.基金项目
国家重点基础研究发展计划（“973”）（2010CB833801），国家自然科学基金重点项目 （）
