生物技术通报Issue(6)：173-180,8.
嗜热绿色糖单孢菌木聚糖酶XyN10A基因在毕赤酵母中的分泌表达及其酶学性质研究
Secretory Expression in Pichia pastoris and Characterization Study of Xylanase from Thermophilic Saccharomonospora viridis
摘要
Abstract
Xylanase has attracted extensive attention because of the application potential in feed, food and paper area, capable of degrading hemicellulose which is prevalent in nature. This research is to construct a recombinant engineered bacteria with efficient secretory expression for thermophilic Saccharomonospora viridis xylanase SviXyN10A using molecular biology techniques, and then study the characterization of the gene product expressed in Pichia pastoris. The results indicate that the gene product of Svixyn10A was successfully expressed in Pichia pastoris, showing the highest enzymatic activity of 7.53 IU/mL with methanol induction for 2 days. Recombinant xylanase SviXyN10A showed good activity at wide range of pH effect(pH5.0-9.0), and the optimum reaction temperature is 70℃, pH7.0. What is more, the xylanase showed excellent thermo-alkali-stability and almost no cellulase activity. Thus, it is a potential candidate for future use in industrial areas particularly in the pulp and paper industry.关键词
绿色糖单孢菌/木聚糖酶/分泌表达/毕赤酵母Key words
Saccharomonospora virid/Xylanase/Secretory expression/Pichia pastoris引用本文复制引用
玉王宁,金一,谢响明,刘伟娜,郑菲,厚凌宇,王晓宇,梁迪,王伟轩,张朔,柳静言..嗜热绿色糖单孢菌木聚糖酶XyN10A基因在毕赤酵母中的分泌表达及其酶学性质研究[J].生物技术通报,2014,(6):173-180,8.基金项目
国家自然科学基金项目（31070635，30870028，J1103516），公益性行业（农业）科研专项（201103027），北京市大学生科学研究与创业行动计划 （）
