生物技术通报Issue(7)：37-43,7.
CRISPR-Cas介导的基因编辑工具
Gene Editing Tools Mediated by CRISPR-Cas
摘要
Abstract
Recently, with the deepening of the research and transformation of CRISPR-Cas system, it has been applied as a new gene targeting modification technology, which can be directed to silence target genes. At present, this technology has been successfully used in HEK293, mouse and zebrafish cells and generated stable gene knockout cell lines, meanwhile gene knockout models has been constructed on the models animals such as mice and fruit flies. With the advantages of simple design, short time-consuming and higher efficiency, CRISPR-Cas has become a gene targeting knockout technology with broad application prospect. In this paper, structure and function mechanism of the CRISPR-Cas, also the current applications of Cas9/gRNA were reviewed.关键词
CRISPR-Cas/基因修饰/基因敲除Key words
CRISPR-Cas/Genetic modification/Gene knockout引用本文复制引用
左其生,李东,张亚妮,李碧春..CRISPR-Cas介导的基因编辑工具[J].生物技术通报,2014,(7):37-43,7.基金项目
国家自然科学基金项目（31272429），江苏省研究生培养创新工程 （）
