生物技术通报Issue(7)：49-53,5.
苹果树腐烂病菌胞外蛋白提取方法
Study on Extracting of Extracellular Proteins in Valsa mali
摘要
Abstract
An optimal protein extraction method, as much as possible to obtain high-quality extracellular proteins of Valsa mali, is helpful to thoroughly analyze the proteomics and pathogenesis of the pathogen. In this study, the three methods freeze drying system/dialysis, DOC-10%TCA precipitation and ammonium sulfate precipitation were used to extract the extracellular proteins of Valsa mali, respectively. Then the optimal concentration of ammonium sulfate needed to be screened. The proteins extracted by the three methods were quantified and separated separately through Bradford and SDS-PAGE, and then extracellular proteins extracted from pathogenicity of different isolates were also compared with SDS-PAGE. The results showed that the content and distinguishability of protein sample extracted by 70%ammonium sulfate was optimal, and four protein lanes are significantly different in the SDS-PAGE electrophoresis patterns of different isolates. So this method is the most suitable for extracting extracellular protein sample to analyse the difference of Valsa mali protein.关键词
苹果树腐烂病菌/硫酸铵沉淀/蛋白定量/聚丙烯酰胺凝胶电泳/差异蛋白Key words
Valsa mali/Ammonium sulfate precipitation/Protein quantification/SDS-PAGE/Different proteins引用本文复制引用
王海英,何媛媛,黄丽丽,韩青梅..苹果树腐烂病菌胞外蛋白提取方法[J].生物技术通报,2014,(7):49-53,5.基金项目
国家自然科学基金项目（31171796），高等学校博士学科点专项科研基金（20120204110002），高等学校学科创新引智计划（B07049），公益性行业（农业）科研专项 （）
