生物技术通报Issue(7)：119-124,6.
基于高通量测序的辽东栎转录组学研究
Transcriptome Analysis for Quercus liaotungensis Koidz. Based on High-throughput Sequencing Technology
摘要
Abstract
In this study, Illumina Solexa Hiseq 2000 high-throughput sequencing technology was used to get the comprehensive transcriptome from mixed samples of buds, flowers, leaves and fruits of Quercus liaotungensis. As a result, 3.8 Gb effective data was obtained. After de novo assembly by the software of Trinity, a total of 95 800 unigenes were generated, corresponding to a total of 73.57 Mb with a maximum length, average length and N50 of 11 284 bp, 768 bp and 1 373 bp respectively. Using Blastx against the public databases of Nr and Swiss-Prot with an E-value cut-off of 10-5, 38 163 unigene were not found in any databases with a high homology. According to the KEGG pathway assignment, 67 unigene encoding nine key enzymes which may involve in starch synthesis were identified. In addition, 15 901 potential SSR loci were detected from 13 380 unigene. Of them, dinucleotide repeat and trinucleotide repeat accounted for 98.16%of all.关键词
辽东栎/Illumina/Solexa/转录组/淀粉/SSRKey words
Quercus liaotungensis/Illumina/Solexa/Transcriptome/Starch/SSR引用本文复制引用
刘玉林,李伟,张志翔..基于高通量测序的辽东栎转录组学研究[J].生物技术通报,2014,(7):119-124,6.基金项目
“十二五”国家科技支撑计划课题（2011BAD22B08），国家林业公益性行业科研专项项目 （）
