生物技术通报Issue(7)：125-130,6.
内蒙古白绒山羊翻译控制肿瘤蛋白（TCTP）基因克隆及组织表达特性分析
Cloning and Tissue-specific Expression of TCTP Gene in Inner Mongolia Cashmere Goat(Capra hircus)
摘要
Abstract
The present study aims at cloning the CDS fragment of Translationally controlled tumor protein(TCTP)gene cDNA in Inner Mongolia Cashmere Goat and analyzing its expression pattern. TCTP gene cDNA was cloned by RT-PCR. The nucleotide sequence was analyzed by BLAST, while amino acid sequence was analyzed by online softwares. The tissue-specific expression pattern of TCTP was analyzed by quantitative RT-PCR. The cloned TCTP gene cDNA was 519 bp in length, including a complete ORF encoding 172 amino acids. The full cDNA nucleotide sequence has an identity from 99%to 95%with Ovis aries, Bos taurus, Sus scrofa, Homo sapiens, Macaca mulatta and Rattus norvegicus. Bioinformatics analysis showed that theoretical molecular mass of encoded protein was 19.6 kD, isoelectric point(pI)was 4.673. It contained a N-glycosylation sites, a protein kinase C phosphorylation sites and three casein kinaseⅡphosphorylation sites and most probably is localized in cytoplasm. The results of quantitative RT-PCR showed that TCTP gene is expressed in kidney, muscle, pancreas, liver, testis and brain. The expression was higher in liver, whereas lower in brain.关键词
内蒙古白绒山羊/翻译控制肿瘤蛋白/基因克隆/表达模式Key words
Inner Mongolia cashmere goat/Translationally controlled tumor protein(TCTP)/Gene cloning/Expression pattern引用本文复制引用
杨立敏,姚睿原,郭志新,朝格图,其布日,郑旭,鲍文蕾,王志钢..内蒙古白绒山羊翻译控制肿瘤蛋白（TCTP）基因克隆及组织表达特性分析[J].生物技术通报,2014,(7):125-130,6.基金项目
国家自然科学基金项目 （）
