生物技术通报Issue(7)：131-136,6.
兔豆状囊尾蚴TpRS1基因的克隆及其序列分析
Cloning and Sequence Analysis of TpRS1 Gene from Cysticercus pisiformis
摘要
Abstract
To study the function of TpRS1 gene from Cysticercus pisiformis, the open reading frame(ORF)cDNA sequence of TpRS1 gene was cloned by RT-PCR from C. pisiformis total RNA with primers derived from Taenia solium RS1 gene sequence in the GenBank database. Biochemical properties, secondary structure, signal, hydrophobicity and antigenicity in TpRS1 protein were predicted by bioinformatics tools. The results indicated that TpRS1 cDNA sequence contained an ORF of 258 nucleotides and the deduced protein consisted of 85 amino acids with the theoretical molecular weight of 9.6 kD and isoelectric point of 9.07. Analysis of secondary structure revealed 70.59%and 5.88%ofα-helix andβ-strands, respectively, and others were loop. The signal peptide sequence of TpRS1 were not identified. TpRS1 protein sequence showed more than 72%identity with other Taenia spp.. Phylogenetic analysis indicated that TpRS1 located in the same clade with Taenia hydatigena.关键词
豆状囊尾蚴/TpRS1基因/克隆/序列分析Key words
Cysticercus pisiformis/TpRS1 gene/Cloning/Sequence analysis引用本文复制引用
韩乐乐,李作磊,苟惠天,孙晓林..兔豆状囊尾蚴TpRS1基因的克隆及其序列分析[J].生物技术通报,2014,(7):131-136,6.基金项目
甘肃省农业生物技术研究与应用开发项目（GNSW-2010-01），甘肃省科技支撑计划项目 （）
