生物技术通报Issue(7)：162-167,6.
一株产阿魏酸酯酶丝状真菌的分离鉴定及其粗酶性质研究
Isolation,Identification and Enzyme Properties of a Strain Producing Feruloyl Esterases
摘要
Abstract
Using the method of transparent zone, 37 filamentous fungi producing feruloyl esterase(FAE)were selected from rot soil samples containing wood fibers. The strain HA4087 was isolated by its high FAE activities. It was identified as Alternaria alternata by conidia morphology, 18S rDNA sequence and ITS sequence analysis. The characteristics of crude feruloyl esterase from A. alternate HA4087 were preliminarily studied. FAE ac tivity of the crude enzyme was 86 mU/mL. The optimal temperature and pH for crude FAE was 55℃and pH5.5, respectively. Crude FAE from A. alternate HA4087 remained nearly 90%of its maximal activity at 70℃and was active in the pH range of 3.0-7.0. The strain producing thermostable and high active FAE provided a prerequisite for the industrialization of feruloyl esterase.关键词
阿魏酸酯酶/菌种鉴定/互隔交链格孢霉/酶学性质Key words
Feruloyl esterase/Identification/Alternaria alternata/Enzyme properties引用本文复制引用
胡泓宇,周燕燕,陈静,侯运华..一株产阿魏酸酯酶丝状真菌的分离鉴定及其粗酶性质研究[J].生物技术通报,2014,(7):162-167,6.基金项目
山东省高等学校科技计划项目 （）
