生物技术通报Issue(7)：179-183,5.
肠道病毒71型（EV71）荧光病毒的构建
The Construction of the EGFP Enterovirus 71
朱守海 1王璐璐2
作者信息
- 1. 天津科技大学生物工程学院，天津 300457
- 2. 天津市国际生物医药联合研究院，天津 300457
- 折叠
摘要
Abstract
Human Enterovirus 71(EV71)is one of the major agents causing hand, foot and mouth disease(HFMD). An infectious full-length cDNA clone of EV71 as well as the recuse virus were obtained using the reverse genetic techniques. Then, the EGFP gene was inserted into the EV71 genome to construct the reporter virus. The results show that the EGFP-EV71 virus not only are replication-competent and infectious, but the EGFP gene are stable during passaging, implicating the recombinant virus could be used as a reporter virus for high throughput EV71 antiviral drugs screening.关键词
EV71/感染性克隆/增强型绿色荧光蛋白/荧光病毒Key words
EV71/Infectious cDNA clone/EGFP/Reporter virus引用本文复制引用
朱守海,王璐璐..肠道病毒71型（EV71）荧光病毒的构建[J].生物技术通报,2014,(7):179-183,5.
