生物技术通报Issue(7)：201-208,8.
蛋白A-葡聚糖-Fe3O4纳米磁珠的制备及对IgG分离纯化
Fe3O4 Magnetic Dextran Nanoparticles Modified with SPA Ligand for IgG Purification
摘要
Abstract
The objective of present research is to prepare magnetic dextran nanoparticles conjugated with Staphylococcal Protein A(SPA-MDPs)for the purification of IgG. The magnetic nanoparticles were prepared by following a high-temperature solution-phase procedure, and the average particle size can be tuned from 30 to 200 nm by simply varying the concentration of NaOH during the reaction. The size of SPA-MDPs nanoparticles has a great influence on their adsorption capacity for IgG. A maximum adsorption of human IgG was calculated to be 84.85 mg/mL with a binding affinity constant of 3.48×106 M-1 when the average size of SPA-MDPs is about 101.5 nm. The experiment results also demonstrated that the SPA-MDPs nanoparticles can be recycled for 5 times with minor loss of adsorption capacity. Moreover, IgG was purified from human plasma with a purity of 93.5%and recovery of 88.7%. Thus, SPA-MDPs show promising potentials for isolation and purification of IgG.关键词
葡聚糖/纳米磁珠/蛋白A/IgG纯化Key words
Dextran/Magnetic nanoparticles/SPA/IgG purification引用本文复制引用
任敬钢,程超,仲从浩,张丽,李荣秀..蛋白A-葡聚糖-Fe3O4纳米磁珠的制备及对IgG分离纯化[J].生物技术通报,2014,(7):201-208,8.基金项目
国家科技重大专项（重大新药创制专项） （）
