生物技术通报Issue(8)：70-75,6.
烟粉虱全基因组DNA四种提取方法的比较
Comparison of Four Methods for Whole Genomic DNA Extraction from Bemisia tabaci
摘要
Abstract
Obtaining high-quality DNA is a prerequisite to study the molecular mechanisms of many organisms. For tiny insects, low DNA concentration(from one single insect)is unable to meet the need of some experiments. To find a convenient and efficient method for whiteflies genome extraction, KAc, salting-out, chloroform-isoamylol and phenol methods were used to extract bull whiteflies genome DNA, respectively. The efficiency of different DNA extraction methods were evaluated by comparing the concentration, purity, PCR products of DNA, extraction time and the toxicity of the reagents, respectively. The results showed that the average concentration of DNA extraction of salting-out method was 521 ng/μL, and the purity could meet the experiment requirements, and the PCR product brand of MSAP primer amplification was bright. Compared to other methods, salting-out method was more simple, rapid and non-toxic. Therefore, salting-out method was the best ideally method to extract the whole genomic DNA from whiteflies.关键词
DNA提取/烟粉虱/KAc法/氯仿-异戊醇法/盐析法/苯酚法Key words
DNA extraction/Bemisia tabaci/KAc method/Chloroform-isoamylol method/Salting-out method/Phenol method引用本文复制引用
戴恬美,吕志创,万方浩..烟粉虱全基因组DNA四种提取方法的比较[J].生物技术通报,2014,(8):70-75,6.基金项目
国家自然科学基金项目（31100269），“973”计划（2009CB119200），公益性行业（农业）科研专项 （）
