生物技术通报Issue(8)：82-88,7.
牦牛PAFR基因生物信息学分析及其在妊娠前后子宫中的表达
Cloning and Sequence Analysis of Yak PAFR Gene and Expression in Uterus Before and After Pregnancy
摘要
Abstract
Platelet-activating factor receptor(PAFR)gene were cloned from uterus of domestic yak by reverse transcription polymerase chain reaction(RT-PCR). The results shows that domestic yak PAFR gene was 1 029 bp in length, encoded predicted mature protein of 342 amino acids, which encoded a hydrophilic protein. Compared with the reference sequence of PCR primer designing homology of the domestic yak PAFR coding sequence was 99.7%at the amino acid level, and also showed high homology of with other species. The PAFR has 5 transmembrane domains, features G protein-coupled receptor domains, no signal peptide. The result of the quantitative Real-time Polymerase chain reaction (QRT-PCR)shows that PAFR was highest expression in gestation and lowest in follicular phase in uterus of domestic yak. That suggested PAFR play an important role in implantation and gestation.关键词
牦牛/PAFR/基因克隆/生物信息学分析/基因表达Key words
Yak/PAFR/Gene cloning/Bioinformatic analysis/Gene expression引用本文复制引用
魏博,潘阳阳,禹尧,田枫,余四九..牦牛PAFR基因生物信息学分析及其在妊娠前后子宫中的表达[J].生物技术通报,2014,(8):82-88,7.基金项目
国家自然科学基金项目 （）
