生物技术通报Issue(8)：89-93,5.
牦牛不同组织TLR3、TLR5 mRNA转录水平相对定量研究
Relative Quantification of mRNA Transcription of TLR3 and TLR5 in Different Tissues of Yak
摘要
Abstract
According to the sequences of yak TLR3 and TLR5, the real-time PCR primers were designed to establish a method for the detection of mRNA transcription of TLRs by Real-time quantitative PCR, and the transcription level of TLRs in different tissues of yak was analyzed. The results showed that two genes have different expression pattern and expression level in tissues. The TLR3 mRNA was detected in examined tissues except mammary gland, and it was expressed at a higher level in heart, large intestine, stomach and muscle. The TLR5 mRNA level was higher in heart, lung, large intestine, small intestine, stomach, muscle and ovary. The results indicated that there exists difference in the transcription level of TLRs in tissues, which may be associated with the recognition of pathogens. For the same gene, the mechanism of gene may bring difference in expression level in different tissues.关键词
牦牛/转录水平/TLRs/相对定量Key words
Yak/Transcriptional level/TLRs/Relative quantification引用本文复制引用
陈亚冰,兰道亮,林宝山,黄偲,黄勇,李键..牦牛不同组织TLR3、TLR5 mRNA转录水平相对定量研究[J].生物技术通报,2014,(8):89-93,5.基金项目
中央高校青年教师基金项目 （）
