生物技术通报Issue(8)：126-131,6.
静水椎螺乙酰胆碱结合蛋白在Bac-to-Bac系统中的表达、纯化与结晶
Expression,Purification,Crystallization of Acetylcholine Binding Proteins from Lymnaea stagnalis in Bac-to-Bac System
摘要
Abstract
It was to construct a recombinant bacmid that expresses acetylcholine binding protein from Lymnaea stagnalis(Ls-AChBP) and express the Ls-AChBP functional genes in Bac-to-Bac expression system to obtain the crystal. Synthesized Ls-AChBP cDNA was inserted into the correct position of the baculovirus expression vector pFastBac1. After the positive recombinant bacmid was screened, the recombinant bacmid was transfected into the insect cells to generate restructured baculovirus for high expression of the recombinant protein Ls-AChBP(rLs-AChBP), which was captured by nickel-charged resin, and further purified by gel filtration chromatography. The rLs-AChBP pentamer was obtained and performed crystallization by robot screen. Recombinant Ls-AChBP expression in Bac-to-Bac system was successfully and we got Ls-AChBP crystal. Ls-AChBP is one of the AchBPs. The crystal structure of rLs-AchBP is an available template of description of nAChRs structure and function.关键词
静水椎螺乙酰胆碱结合蛋白/Bac-to-Bac杆状病毒表达系统/重组表达/纯化/结晶Key words
Ls-AChBP/Bac-to-Bac expression system/Recombinant expression/Purification/Crystallization引用本文复制引用
林波,孟海玲,吴勇,邴晖,长孙东亭,罗素兰..静水椎螺乙酰胆碱结合蛋白在Bac-to-Bac系统中的表达、纯化与结晶[J].生物技术通报,2014,(8):126-131,6.基金项目
国家自然科学基金项目（41366002），国家国际科技合作专项（2011DFR31210），海南省社会发展科技专项（SF201328），海口市重点科技计划项目（2013-16），长江学者和创新团队发展计划（IRT1123），海南省研究生创新科研课题 （）
