生物技术通报Issue(8)：138-145,8.
不同品种香蕉内生菌分离及广谱拮抗菌的筛选
Endophytes Isolation and Broad-spectrum Antagonistic Bacterias Screening from Banana
摘要
Abstract
In order to determine the main distribution of endophytes and their broad-spectrum antimicrobial activity, endophytes were obtained from healthy and diseased tissues of two disease-resistant and one disease susceptible banana cultivars. Endophytes were separated from roots, corms, pseudostems, leaves and store in the ultra-low on Luria-Bertani(LB), Yeast Extract with supplements(YE), and Potato Dextrose Agar(PDA)strain store medium. Then screened broad-spectrum antagonistic bacteria which against Fusarium oxysporum f. sp. Cubense, Curvularia lunata, Curvularia fallax, Corynespora cassiicola(Berk&Curt)Wei, Alternaria musae, Deightoniella troulosa, Colletotrichum musae, Pestalogiopsis sp., Btoryosphaeria dothidea. Taxonomy identification of 041, 04-1, 19-1, 03A-1 was conducted by evaluating morphologic characteristics and 16S rDNA gene sequences for phylogenetic analysis. After purification, total of 438 endophytes were obtained. The total of isolates showed that we obtained 240 strains bacteria, followed by 142 strains actinomycetes, and 56 strains fungi. The richest number of endophytes that isolated from diseased NanTian banana cultivars(128). Ten actinomyces and two bacterias were determined to possess antibiotic activity against Ten banana pathogens. Isolates 041 was the most effective and had 28.13±1.89 mm width of inhibition zone. Isolated 041, 04-1, 19-1, 034-1 were identified as Streptomyces misionensis.关键词
香蕉/内生菌/广谱拮抗菌/分离Key words
Banana/Endophytes/Broad-spectrum antagonistic bacteria/Isolation引用本文复制引用
王梦颖,周登博,井涛,胡一凤,高祝芬,谢晴宜,张锡炎,戚春林..不同品种香蕉内生菌分离及广谱拮抗菌的筛选[J].生物技术通报,2014,(8):138-145,8.基金项目
现代农业产业技术体系建设项目专项 （）
