生物技术通报Issue(8)：169-174,6.
响应面法优化解淀粉芽孢杆菌C101发酵培养基
Optimization of Fermentation Medium by Bacillus amyloliquefaciens Strain C101 Using Response Surface Methodology
摘要
Abstract
The fermentation medium components for spore content by Bacillus amyloliquefaciens strain C101 was optimized by response surface methodology(RSM). Firstly, three of the most significant influence factors were screened by the method of Plackett-Burman design as MnSO4, KH2PO4 and(NH4)2SO4. The path of steepest ascent was applied to approach the optimal region of the three significant factors. Lastly, the optimal concentration and correlations among three factors were identified by RSM. The optimal fermentation conditions were determined as followed:sucrose 20 g/L, urea 4.0 g/L, soybean meal 4.0 g/L, KNO3 2.0 g/L, Na2HPO4 2.4 g/L, KH2PO4 0.52 g/L,(NH4)2SO4 0.55 g/L, NaCl 1.0 g/L, MgSO4·7H2O 0.50 g/L, FeSO4 0.005 0 g/L, MnSO4 0.005 4 g/L. Under these conditions, the maximum theoretic spore number was 14.67×108/mL. After three parallel verifications, the maximum theoretic value was consistent with mean value of verification test and the spore number was increased by 188%compare to that before optimization.关键词
响应面法/解淀粉芽孢杆菌/芽孢/优化Key words
Response surface methodology(RSM)/Bacillus amyloliquefaciens/Spore Optimization引用本文复制引用
梁昌聪,郭立佳,刘磊,张建华,杨腊英,王国芬,黄俊生..响应面法优化解淀粉芽孢杆菌C101发酵培养基[J].生物技术通报,2014,(8):169-174,6.基金项目
国家公益性行业（农业）科研项目 （）
