生物技术通报Issue(8)：202-207,6.
食源性沙门氏菌耐药性及质粒介导喹诺酮耐药基因检测
Study on Antimicrobial Resistance and Plasmid-mediated Quinolone Resistance of Foodborne Salmonella Isolates
摘要
Abstract
A total of 638 food samples were collected randomly to determine the prevalence of Salmonella. The overall percentage of Salmonella prevalence was 9.7%(n=62). Among 16 different serotypes identified, S. Anatum was most common. The isolates were frequently resistant to sulfamethoxazole, trimethoprim/sulfamethoxazole, streptomycin, and ciprofloxacin. Five different types of amino acid substitutions were identified in quinolone resistance-determining region(QRDR)of GyrA and ParC of 16 ciprofloxacin-resistant isolates. Double mutations of Ser83Phe and Asp87Gly in GyrA accompanied by an additional mutation of Ser80Arg in ParC were found most frequently. The qnr genes were present in seven(11.3%)of 62 isolates, and among them, two isolates carried qnrA and five carried qnrS. qnrB, qnrC, and qnrD were not detected in the isolates in this study. Eight isolates were positive for aac(6')-Ib, of which three isolates carried the-cr variant. The results suggested the diversity of serotype distribution, serious situation of antimicrobial resistance and the presence of plasmid-mediated quinolone resistance(PMQR)genes in foodborne Salmonella from Xinxiang.关键词
食源性/沙门氏菌/耐药性/质粒介导/喹诺酮类Key words
Foodborne/Salmonella/Antimicrobial resistance/Plasmid-mediated/Quinolone引用本文复制引用
刘贵深,于涛..食源性沙门氏菌耐药性及质粒介导喹诺酮耐药基因检测[J].生物技术通报,2014,(8):202-207,6.基金项目
河南省教育厅科学技术研究重点项目 （）
