生物技术通报Issue(11)：187-192,6.
一株产纤维素酶细菌的分离鉴定及酶学特性研究
Isolation,Identification and Enzymatic Characterization of a Cellulase-Producing Bacteria
摘要
Abstract
A cellulase-producing bacteria strain JJ-3 with high activity was isolated from the soil containing rotted leaves, which was identified asKlebsiella oxytoca based on the 16S rRNA sequence analysis. The optimal enzyme-producing conditions of strain JJ-3 was investigated and was as follows:filter paper as carbon source, peptone as nitrogen source, pH8.0, three-day fermentation. And the fermentation culture had higher FPase activity under neutral and alkaline conditions, with 118.7 U/mL(pH7.0), 167.8 U/mL(pH8.0), 120 U/mL(pH9.0). The results of enzymatic characterization showed that the optimal pH and temperature for enzyme reaction was 7.0 and 40℃, respectively. FPase was sensitive to temperature, while it kept good stability during the pH7.0 to 8.0. This study suggested that the cellulase from strain JJ-3 meets the request of neutral and alkaline cellulase.关键词
纤维素酶/产酸克雷伯氏杆菌/产酶条件/酶学特性Key words
Cellulase/Klebsiella oxytoca/Enzyme-producing conditions/Enzymatic characterization引用本文复制引用
贾博涵,周伟,赵罗迪,杨埔,苟敏..一株产纤维素酶细菌的分离鉴定及酶学特性研究[J].生物技术通报,2014,(11):187-192,6.基金项目
国家自然科学基金项目 （）
