生物技术通报Issue(11)：214-218,5.
重组酵母产人IFN-α2a与LL-37融合蛋白的工艺优化
The Process Optimization ofPichia pastoris GS115LI for Producing Fused Protein of LL-37 and IFN-α2a
摘要
Abstract
With experiments of single factor, the optimal process of recombinantP. pastoris GS115LI for producing fused protein of LL-37 and IFN-α2a were using BMMY as inducing medium, inoculum as OD600=5.5, temperature as 26℃, pH6.0, inducing chemical(methanol) adding amount as 1.0% every 24 h, incubating shaking speed as 200 r/min, and inducing time as 144 h. Under those optimal conditions, the 27.9 mm of LL-37 antimicrobial activity could be gotten. Compared with the original conditions, the productivity of fused protein of LL-37 and IFN-α2a was increased as 32.29%.关键词
干扰素α2a/LL-37/工艺Key words
Interferon-α2a/LL-37/Fermentation process引用本文复制引用
张明杰..重组酵母产人IFN-α2a与LL-37融合蛋白的工艺优化[J].生物技术通报,2014,(11):214-218,5.基金项目
广东省重大科技专项 （）
