生物技术通报Issue(1)：92-97,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.01.014
甘蔗转基因甘露糖筛选系统的建立
Establishment of Mannose Selection System in Sugarcane Transformation
摘要
Abstract
In this research the killing curve of mannose was tested at the subculture,differentiation and rooting stages of sugarcane cultivar ROC22. Then the expression cassette containing a pmiselection marker gene and a GFP report gene was transformed into sugarcane embryonic callus byAgrobacterium-mediated method. Resistant plants were obtained after selection by Mannose. The PCR detection result and GFP microscope observation result both show that our lab had established a high efficiency PMI/Mannose selection system in sugarcane transformation.关键词
甘蔗转基因/甘露糖/pmi基因/GFP基因Key words
sugarcane transgenic/mannose/pmi gene/GFP gene引用本文复制引用
王文治,杨本鹏,蔡文伟,冯翠莲,王俊刚,熊国如,张树珍..甘蔗转基因甘露糖筛选系统的建立[J].生物技术通报,2015,(1):92-97,6.基金项目
现代农业产业技术体系建设专项资金项目（CARS-20-2-5），Cry1Ac-2A-gna融合基因遗传转化甘蔗的研究项目 （）
