生物技术通报Issue(1)：98-103,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.01.015
乌腺金丝桃愈伤组织中总黄酮及金丝桃素含量测定
Determination the Content of Total Flavonoids and Hypericin in Callus fromHypericum attenuatumChoisy
刘晓丹 1张克勤 2刘连 3李文昌1
作者信息
- 1. 吉林农业科技学院生物工程学院，吉林 132101
- 2. 长白山动植物资源利用与保护吉林省高校重点实验室，吉林 132101
- 3. 长白山动植物资源利用与保护吉林省高校重点实验室，吉林 132101
- 折叠
摘要
Abstract
With fresh leaves as test material, this study established a relatively stable system of callus culture forHypericum attenuatum Choisy, and determined the content of total flavonoids and hypericin in callus. With MS as the basic culture medium, the effects of different hormone combinations were investigated on callus induction. The results showed that the optimum culture media for callus induction was MS + 2,4-D 4.0 mg/L + 6-BA 0.2 mg/L. The total flavonoids and hypericin of callus were extracted using ultrasonic method, respectively, using UV spectrophotometry and HPLC method for determination total flavonoids content and hypericin content in callus. The results showed that total flavonoids was 27.99 mg/g, and hypericin content was 0.515 mg/g in dry callus.关键词
乌腺金丝桃/愈伤组织/诱导/总黄酮/金丝桃素Key words
Hypericum attenuatumChoisy/callus/induction/total flavonoids/hypericin引用本文复制引用
刘晓丹,张克勤,刘连,李文昌..乌腺金丝桃愈伤组织中总黄酮及金丝桃素含量测定[J].生物技术通报,2015,(1):98-103,6.
