生物技术通报Issue(2)：148-152,5.DOI:10.13560/j.cnki.biotech.bull.1985.2015.02.022
有性杂交选育银耳优良菌株的初步研究
Sexual Hybridization Breeding Selection for Good Quality Strain of Tremella fuciformis
摘要
Abstract
During the process of Tremella fuciformis cultivation, some problems, such as low yield and long cultivation time, often are followed with different strains. The aim of this study is to mix good properties of different strain together to new strains by sexual hybridization method. Based comparison of yield, fresh ear ventral color and mature culture, yinke 1# and Tr21 were selected as parents strains with good properties. 50 single spores were isolated from fresh ear and partnered each other following single hybridization principle. 17 combinations are identified with an optical microscope examination and 7 genetic characteristics of hybrid strains are different from parent strains by Antagonism experiment. The results of agronomic traits comparison showed that cultivation period, diameter and the yield of hybrid strains GT3 are significantly different from the parents, and the production has exceeded the parent strains more than 3%. GT3 can be used as an excellent hybrid strains reserves and has the potential to develop into a new species. Sexual hybridization breeding would be effective way to select good quality strain.关键词
银耳/单孢分离/有性杂交/优良菌株Key words
Tremella fuciformis/single spore isolation/sexual hybridization/good quality strain引用本文复制引用
贺元川,陈仕江,杨勇,贺宗毅,张德利,柴佳炎..有性杂交选育银耳优良菌株的初步研究[J].生物技术通报,2015,(2):148-152,5.基金项目
重庆市科技攻关项目 （）
