生物技术通报Issue(2)：202-207,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.02.030
纤维堆囊菌SoF5-76产埃博霉素B分批发酵动力学研究
Kinetics of Batch Fermentation of Epothilone B by Sorangium cellulosum SoF5-76
摘要
Abstract
The fermentation system produced epothilone is complex, and the production strain is more difficult to operate, leading to difficulties in fermentation parameters measured, so far there is no reporting on Epothilone fermentation kinetics. In this paper, the batch fermentation kinetics of the epothilone B produced by Sorangium cellulosum SoF5-76 was studied in a 5 L fermentor. During the process, the temperature and pH were almost constant, 30℃, 7.4. When the fermentation finished, the dry cell weight, epothilone B production and residual glycerol could reach 3.00 g/L, 18.20 mg/L and 0.049 g/L. On the bases of Logistic and Luedking-Piret equation, the models of cell growth, epothilone B production and substrate consumption were established, and the non-line fitting was employed to model the kinetics of fermentation by software MATLAB. The results show that the kinetic models were in good agreement with the fermentation process.关键词
纤维堆囊菌/埃博霉素B/分批发酵/动力学模型/MATLAB/非线性拟合Key words
Sorangium cellulosum/epothilone B/batch fermentation/kinetic models/MATLAB/non-line fitting引用本文复制引用
马利云,龚国利,王娜..纤维堆囊菌SoF5-76产埃博霉素B分批发酵动力学研究[J].生物技术通报,2015,(2):202-207,6.基金项目
国家自然科学基金项目（20906058），陕西科技大学学术骨干培育项目 （）
