生物技术通报Issue(3)：115-120,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.04.016
甘蔗脱毒健康种苗中单糖转运蛋白基因差异表达分析
The Differential Expression of Monosaccharide Transporter Genes in Disease-free Sugarcane Plants
摘要
Abstract
The sugarcane yield can be increased by planting disease-free sugarcane seedlings. However, the physiological and molecular mechanisms of this yield increasing are still not clear now. Monosaccharide transporters are one type of key regulatory proteins involved in carbon accumulation and partitioning processes, and play an important role in plants defense process. The differential expression of three monosaccharide transporter genes(SGT1,SGT2 andPST2a)in disease-free and untreated sugarcane plants was studied to clarify the potential function of these genes on sugarcane yields. The expression levels ofSGT1,SGT2 andPST2a in leaves of disease-free plants at seedling and tillering stages were obviously higher than untreated plants. Furthermore, the expression levels of these genes were increased in immature internodes of disease-free plants at elongation and mature stages, but there were no difference in mature internodes. The sugarcane leaves(at seedling and tillering stages) and the immature internodes(at elongation and mature stages)grow rapidly and demand for more monosaccharide to provide energy and the precursor of biosynthesis. The up-regulations of these monosaccharide transporter genes might accelerate the growth and biomass accumulation of disease-free sugarcane plants by increasing the transporting and uptake of monosaccharide.关键词
甘蔗/健康种苗/单糖转运蛋白基因/基因表达/产量Key words
sugarcane/disease-free plants/monosaccharide transporter gene/gene expression/yield引用本文复制引用
王俊刚,赵婷婷,杨本鹏,蔡文伟,张树珍..甘蔗脱毒健康种苗中单糖转运蛋白基因差异表达分析[J].生物技术通报,2015,(3):115-120,6.基金项目
“863”计划（2013AA102604-1），海南省自然科学基金项目（313078），海南省重大科技项 （）
