生物技术通报Issue(3)：191-198,8.DOI:10.13560/j.cnki.biotech.bull.1985.2015.04.028
异育银鲫GABA A受体γ2亚基全长克隆及生物信息学分析
Cloning and Bioinformatics Analysis of GABAA Receptorγ2 Subunit Gene inCarassais auratus gibebiol
摘要
Abstract
The application of the specificity of GABA receptor subunits in drug screening and drug development got widely attention recently, of which three function subunitsα1,β2 andγ2 studied deeply. Furthermore,Carassais auratus gibebiolas its good growth and reproduction had been widely farmed in china. In the present study, we cloned and characterized GABA A receptorγ2 subunit full-length gene. The full-lengthCarassais auratus gibebiolGABA A receptorγ2 subunit cDNA was 2 763 bp in length, contained a 1437 bp open reading frame (ORF), and encoded 477 amino acids which constituting a 55.3 kD protein molecule with an isoelectric point of 9.13. Theγ2 subunit protein was hydrophilic. The protein sequence had one signal peptide, consisting of 35 amino acid residue. The sequence of amino acids contained four transmembrane regions, which length of 23,20,23 and 23 aa, involving in electronic transfer catalysis between the internal and external membrane. We found three N-glycosylation sites, two O- glycosylation sites and an extracellular domain which had obvious ligand-gated ion channels’ characteristics. Sequence comparison revealed that the similarity of theγ2 subunit protein all above 89% with other aquatic animals showed that it belongs to GABA A receptor subunits’ family. We conducted a phylogenetic analysis using the neighbor joining(NJ)method. The evolutionary tree showed that theγ2 subunit protein was clustered with the zebra fish which indicated that they are the two most closely related species.关键词
异育银鲫/GABA A受体γ2亚基/克隆/生物信息学分析Key words
Carassais auratus gibebiol/GABA-A receptorγ2 subunit/cloning/bioinformatics analysis引用本文复制引用
赵依妮,胡鲲,孙琪,杨先乐,阮记明,周爱玲..异育银鲫GABA A受体γ2亚基全长克隆及生物信息学分析[J].生物技术通报,2015,(3):191-198,8.基金项目
国家科学自然基金项目（31172430），“863”计划项目（2011AA10A216），公益性农业行业专项项目 （）
