生物技术通报2015，Vol.31Issue(5)：54-60,7.DOI:10.13560/j.cnki.biotech.bull.1985.2015.05.009
利用基因组改组技术选育阿维拉霉素高产菌
Screening and Breeding Strains Producing High-yield Avilamycin by Genome Shuffling
摘要
Abstract
In order to screen strain producing high-yield avilamycin by mutagenesis, a wild-type strainStreptomyces viridochromogenes Tü57 was treated by a series of traditionally physical and chemical mutagenesis, resulting in the acquisition of numbers of forward mutants while judged by the great improvement in avilamycin A production. Genome shuffling was then carried out to fuse different sources of forward mutants, following with various fusants harvestry. The screening strategy adopted the combination method of thin layer chromatography (TLC) and cylinder-plate (two dosages technique) as the primary test, and high performance liquid chromatography (HPLC) as the secondary test. A mutant producing high-yield avilamycin, designated as R708, was obtained. The yield of avilamycin A from this mutant reached to 0.208 g/L in the shake flask experiment, which was a 20-fold increment compared to that of the wild-type strain. Our conclusion is that genome shuffling could increase the genetic diversity of the offspring strains; therefore this method is more quickly and efficiently than traditional mutagenesis in breeding strains producing high-yield avilamycin.关键词
绿色产色链霉菌Tü57/阿维拉霉素/诱变/基因组改组Key words
Streptomyces viridochromogenesTü57/avilamycin/mutagenesis/genome shuffling引用本文复制引用
毛灵琪,李存治,陶兴无,闫达中..利用基因组改组技术选育阿维拉霉素高产菌[J].生物技术通报,2015,31(5):54-60,7.基金项目
湖北省自然科学基金项目(2008CDB067) （2008CDB067）
